Activity following lights-off at CNR, JAX and KI.

A-C Average response per cage across weeks to lights-off in cages (n = 5; individual traces in color) of female mice housed at the three sites. Traces start at light-off (abscissa, minutes) and ends at lights on (dark-cycle is 720 minutes). Each trace corresponds to one cage and is the average activity across multiple weeks normalized to peak activity (= 1.0). The plots deriving from the different sites show clear differences with a short time-to-peak latency at CNR (Table 4), while mice at JAX (B; Table 4) show a bi-phasic response with an early short latency time-to peak followed by a decrease in activity and a second peak at about 400 minutes. At KI (C, Table 4) there is more protracted onset of activity and the time to peak matches the second peak observed at JAX, i.e. 400 minutes after lights-off. The late-night pattern of activity, >400 minutes, show less difference across sites (see also Fig 8).