Additional file 1: of Focal adhesion kinase (FAK) activation by estrogens involves GPER in triple-negative breast cancer cells

Figure S1. GPER stimulation triggers FAK Y397 activation in SUM159 TNBC cells. Immunoblots showing FAK phosphorylation in SUM159 cells treated for 30 min with 100 nM E2 (A) or 100 nM G1 (B) alone or in combination with 100 nM GPER antagonist G-15. Side panels show densitometric analysis of the immunoblots normalized to the loading control. Immunoblots showing FAK phosphorylation in SUM159 cells treated for 30 min with 100 nM E2 (C) or 100 nM G1 (D) alone and in combination with 1 μM FAK kinase inhibitor VS-4718. Side panels show densitometric analysis of the immunoblots normalized to the loading control. FAK expression was used as loading control for pFAK. Results shown are representative of at least three independent experiments. (*) indicates p < 0.05 (TIF 1732 kb