The Hsp90 chaperone system supports and wires the proteome of the transformation program.

(A) Scheme of the transformation protocol and of the major proteomic changes; Reactome pathways defined by proteins that are significantly changed in Rc relative to N cells are indicated. (B) Venn diagrams showing the number of proteins of the Rc-specific program that are affected by Hsp90 inhibitors. The colors red and blue indicate upregulated and downregulated proteins. Note that the indicated percentages are calculated by dividing the number of proteins in the intersect by 697 and 630 for the Venn diagrams on the left and on the right, respectively (see panel A), and that in the bar graphs, the values for the combined treatments with all three inhibitors are hypothetical. (C) Topological features of the Rc transformation-enriched network. The effects of the pharmacologically induced depletion of proteins is compared with the effect of removing the same number of proteins, but chosen randomly, in five independent replicates (average effect of five different random lists). Each group of data points is fitted to a power-law. (D) Functional PPI complexes present in Rc cells are disconnected by Hsp90 inhibition. For the purpose of illustrating the impact of Hsp90 inhibition, it is assumed that affected proteins are completely removed.