Relationship between the number of <i>Cronartium ribicola</i> (A), <i>Melampsora medusae</i> f. sp. <i>deltoidae</i> (B) and <i>Melampsora larici-populina</i> (C) spores from which DNA was isolated and C_t values, as determined by real-time PCR.

Each dot represents a biological independent replicate (corresponding to a known amount of spores), which was obtained by averaging the Ct values of three technical real-time PCR replicates.