Lipoplex size, polydispersity, zeta potential and siRNA entrapment efficiency remains unchanged with long term storage at various temperatures except the lipoplex size and the polydispersity at room temperature.

(A) Size, (B) polydispersity and (C) zeta potential of the resultant lipoplex were measured using a Zetasizer Nano ZS following appropriate dilution in distilled water. Formulations were stored at either -20°C, 4°C or room temperature (RT) and 2 separate batches were made for each condition. (n = 2). (D) siRNA entrapment efficiencies were determined using the Quant-iT PicoGreen reagent. Tests were performed on freshly prepared lipoplex (F–Black shaded bars), and lipoplex stored at various temperatures for different lengths of time post manufacture. Bar graphs represent the mean and the error bars represent the standard deviation. **p value < 0.005, ***p value < 0.0005, ****p value <0.0001, two-way ANOVA using a Dunnett's post-hoc analysis against freshly prepared lipoplexes.