Perturbation in number and size of pavement cells in leaves with altered activity of miR319-targeted <i>TCP</i> genes.

(A) and (B) Mature first leaves (A, left), average area of their lamina (A, right), and number (B) of their abaxial pavement cells. Sample size (N), 10–12. Scale bar, 2 mm. (C) and (D) Images of mature first leaves (C, left) from plants grown in the absence (M, Mock) or presence (DEX) of 12 μM dexamethasone and average area of their lamina (C, right), and number (D) of their abaxial pavement cells. N, 12–15. Scale bar, 2 mm. Col-0;GR, jaw-D;GR, 35S;GR and 35S;sGR indicate Col-0;ProTCP4:mTCP4:GR; jaw-D;ProTCP4:mTCP4:GR, Col-0;Pro35S:mTCP4:GR and Col-0;Pro35S:TCP4:GR, respectively. (E) and (F) Mature leaf area (E) and number of pavement cells (F) from DEX-treated Col-0;ProTCP4:mTCP4:GR and jaw-D;ProTCP4:mTCP4:GR plants relative to respective mock-treated values. Note that the relative value for jaw-D;ProTCP4:mTCP4:GR is smaller than that of Col-0;ProTCP4:mTCP4:GR since the denominator (mock value) for the former is more than that for the latter. (G) Average pavement cell area of leaves shown in (A). (H) and (I) Outline of the abaxial pavement cells (H) of leaves shown in (C) and their average area (I). Scale bar, 100 μm. For (G) and (I), total 125–150 cells per leaf at different regions were measured and averages from 5–8 leaves are shown. (J) and (K) Frequency distribution of pavement cell size in mature first leaves based on their size, of indicated genotypes (J), and of jaw-D;ProTCP4:mTCP4:GR plants grown without (Mock) or with (DEX) 12 μM dexamethasone (K). (L) Mature rosette leaves from 32-day old jaw-D;ProTCP4:mTCP4:GR plants grown in mock control (Mock), in continuous 12 μM dexamethasone (DEX), in dexamethasone for first 10 days and then transferred to mock (D→M), and in mock for first 10 days and then transferred to dexamethasone (M→D). The numbers indicate leaf positions. Scale bar, 1 cm. Error bars, * and ns indicate SD, p t-test was used to determine significant differences.