Effect of paternal obesity on the embryo development on day 4 and 5 of culture.

(A) Picture of a compacting embryo. (B) Boxplots showing the embryo compaction rate in the different groups. (C) Picture of a blastocyst. (D) Boxplots showing the blastulation rate in the three different groups. (E) Blastocyst grading system used (expansion degree from 1 to 4, inner cell mass (ICM) quality from 1 to 3, and trophectoderm (TE) quality from 1 to 3). (F) Boxplots showing the degree of blastocoel expansion at 115h in the different groups. (G) Boxplots showing the ICM quality at 115 h in the three different groups. (H) Boxplots showing the TE quality at 115h in the different groups. Data are expressed as median ± interquartile range. Stars indicated groups that are statistically significantly different from the lean group (*p<0.05, **p<0.01, ***p<0.001).