Four examples of electropherograms (EPGs) of total RNA samples analyzed on 2200 TapeStation.

In this figure, x-axis represents the number of base pairs. The position of other peaks is derived from the position of the first peak (lower marker). Peaks 18S a 28S represent ribosomal (rRNA) subunits. Peak in the position of ~80 bp represents short RNA species and the last peak in the position of 15–16 kbp represents genomic DNA (gDNA). A) EPG of GBM-1 contains proportional peaks for 18S and 28S subunits indicating high integrity of the sample and no visible peak in the position of 15–16 kbp signifying no gDNA contamination. B) EPG of GBM-2 indicates low integrity of the RNA sample judging by the severe degradation of 28S and resulting disproportionality of height of both rRNA peaks. Again, gDNA was not observed. C) EPG of NonT-1 depicts 18S and 28S peaks with approximately same height which, again, is the mark of advanced degradation. Similar abundance of gDNA was also observed. D) EPG of NonT-3 shows fairly intact RNA with minor but visible gDNA contamination.