(A) Impact of mBFP on different nicotinamide cofactors. (B) Effect of non-NADPH nicotinamide cofactors on the fluorescence level of mBFP-NADPH complexes. (C) Dependency of fluorescence signal on the quantity of NADPH (pmol/200 μl in each well) upon the addition of different concentrations of mBFP. (D) Stability of fluorescent signals after the addition of mBFP to solutions containing different amounts of NADPH. Note that the error bars may not be visible if their sizes are smaller than those of symbols.</p
Analyses of intracellular NADPH concentrations are prerequisites for the design of microbial product...
<p>(A) △ 85% Power (Correct); ▲ 85% Power (Unidentified); ◇ 70% Power (Correct); ◆ 70% Power (Uniden...
sFCS measurements were performed ≈16 h after transfection, using a laser power of 1.5 μW. (A): Box p...
(A) Schematic representation of the interaction between NADPH and mBFP followed by immediate enhance...
<p>Change of the protein fluorescence intensity (in percentage) upon addition of cofactor is shown. ...
The reduced form of nicotinamide adenine dinucleotide phosphate (NADPH) functions as a reducing agen...
Four substrates including G6P, lactate, isocitrate, and malate were individually added to cell lysat...
The chemical stability of nicotinamide adenine dinucleotide coenzyme (NADH/NAD(+)) and its derivativ...
With the increasing use of fluorescence-based assays in high-throughput screening (HTS), the possibi...
The fluorescent emission spectra for 5×10-6 M p-aminohippuric and p-aminobenzoic acids in mixtures o...
CHO-K1 cells were transfected with plasmids coding for the monomeric cytosolic FPs (mEGFP(1x), mNeon...
<p>(A) Changes of fluorescence intensity on SGM-PDA with various concentrations of BNF (0.09375, 0.1...
<p>Influence of pH on the fluorescent intensity of the complex C = 2.00 µg/mL, C<sub> β-CD</sub> = 1...
The members of the cofactor family of nicotinamide adenine dinucleotides are involved in many biolog...
Figure S1. Fluorescence excitation and emission spectra of NBD-R595 in aqueous buffer Figure S1. Exc...
Analyses of intracellular NADPH concentrations are prerequisites for the design of microbial product...
<p>(A) △ 85% Power (Correct); ▲ 85% Power (Unidentified); ◇ 70% Power (Correct); ◆ 70% Power (Uniden...
sFCS measurements were performed ≈16 h after transfection, using a laser power of 1.5 μW. (A): Box p...
(A) Schematic representation of the interaction between NADPH and mBFP followed by immediate enhance...
<p>Change of the protein fluorescence intensity (in percentage) upon addition of cofactor is shown. ...
The reduced form of nicotinamide adenine dinucleotide phosphate (NADPH) functions as a reducing agen...
Four substrates including G6P, lactate, isocitrate, and malate were individually added to cell lysat...
The chemical stability of nicotinamide adenine dinucleotide coenzyme (NADH/NAD(+)) and its derivativ...
With the increasing use of fluorescence-based assays in high-throughput screening (HTS), the possibi...
The fluorescent emission spectra for 5×10-6 M p-aminohippuric and p-aminobenzoic acids in mixtures o...
CHO-K1 cells were transfected with plasmids coding for the monomeric cytosolic FPs (mEGFP(1x), mNeon...
<p>(A) Changes of fluorescence intensity on SGM-PDA with various concentrations of BNF (0.09375, 0.1...
<p>Influence of pH on the fluorescent intensity of the complex C = 2.00 µg/mL, C<sub> β-CD</sub> = 1...
The members of the cofactor family of nicotinamide adenine dinucleotides are involved in many biolog...
Figure S1. Fluorescence excitation and emission spectra of NBD-R595 in aqueous buffer Figure S1. Exc...
Analyses of intracellular NADPH concentrations are prerequisites for the design of microbial product...
<p>(A) △ 85% Power (Correct); ▲ 85% Power (Unidentified); ◇ 70% Power (Correct); ◆ 70% Power (Uniden...
sFCS measurements were performed ≈16 h after transfection, using a laser power of 1.5 μW. (A): Box p...