<i>Tg</i>ApiAT5-3 mediates the uptake of L-tyrosine and L-phenylalanine into <i>T</i>. <i>gondii</i>.

Initial rate of uptake of (A) [14C]Tyr, (B) [14C]Phe, and (C) [14C]Arg, in WT (RHΔhxgprt/Tomato), apiAT5-3Δ188–504, and apiAT5-3Δ188-504/cTgApiAT5-3 strain parasites. Uptake was measured in PBS-glucose containing either 60 μM unlabelled L-Tyr and 0.1 μCi/ml [14C]Tyr (A), 15 μM unlabelled L-Phe and 0.1 μCi/ml [14C]Phe (B), or 100 μM unlabelled L-Arg and 0.1 μCi/ml [14C]Arg (C). The initial rates of transport for each substrate were computed from the initial slopes of the fitted single-order exponential curves (S8 Fig), and represent the mean ± SEM from three independent experiments (* PPt test).