Analysis of [¹³C] amino acid uptake into WT and <i>apiAT5-3</i>^{<i>Δ188–504</i>} parasites reveals a role for <i>Tg</i>ApiAT5-3 in amino acid homeostasis.

(A-B) Extracellular WT (TATi/Tomato) or apiAT5-3Δ188–504 tachyzoites were incubated in medium containing [13C]-L-amino acids for 15 min. Polar metabolites were extracted and amino acid abundance (A) and levels of [13C]-amino acid enrichment (B) in WT (black) and apiAT5-3Δ188–504 (red) tachyzoites determined by GC-MS. Only L-amino acids that could be detected in all experiments are shown. The data are averaged from three independent experiments and error bars represent ± s.e.m. (*, P t test. Where significance values are not shown, the differences were not significant; P > 0.05).