Actin structure measurements.

A) depicts the phalloidin (green) and DAPI (blue) staining of LN229 control cells on the left and the respective structure image of the actin cytoskeleton as a heat map on the right. A correspondence of highly structured regions in the actin staining with the respective structure image is visible. Furthermore, unstructured, homogeneous regions do not contribute to the structure image, as for example in the center of the image. The scaling corresponds to 20 μm. B) shows the quantification of the density of actin structures for U87 cells treated with cannabinoid receptor agonists and inverse agonists as mean value with the sem. C) and D) illustrates the density of actin structures in LN229 and U138 cells after cannabinoid treatment. Various effects that show no apparent receptor specificity were observed. Statistics was performed using a Kruskal-Wallis test and significance was chosen for p<0.05. The asterisk denotes significant results regarding the respective measurement indicated with the bar.