SMP trajectory statistics.

(A-I) Mouse fibroblast cell trajectories with a normalized origin on wrinkled (W; A-C), non-wrinkled (NW; D-F) and active (A; G-I) substrates for each time region. Trajectories have been rotated so that the x-axis corresponds with the wrinkle direction. Cells on static non-wrinkled substrates explore space equally in all directions, while cells on static wrinkled substrates show preferred motion along the wrinkle direction. (J) Ratio of the components of the radius of gyration tensor along (R_xx) and perpendicular to (R_yy) the direction of wrinkles, quantifying the degree of anisotropy in cell trajectories on non-wrinkled (blue), wrinkled (green) and active (red) substrates. Cells on non-wrinkled substrates explore space equally in all directions, while cells on wrinkled and active substrates prefer motion along substrate wrinkles. (K) The ratio of the x and y components of mouse fibroblast velocities also quantifies the anisotropy of cell trajectories, averaged over all times. Cells on wrinkled and active substrates have significantly higher speeds along wrinkles, while cells on non-wrinkled substrates have approximately equal speeds in all directions. Single asterisks (*) indicate significance levels below 0.05, while double asterisks (**) indicate levels below 0.01. There were approximately 103 cells per substrate type across 3 technical replicates and 3 biological replicates.