Analysis of the GpiB, SspM, SspN and SspO secretion signals in a <i>S</i>. <i>cerevisiae</i> complementation assay.
- Berit Hassing (6331109)
- David Winter (6331112)
- Yvonne Becker (2396650)
- Carl H. Mesarich (3805033)
- Carla J. Eaton (300796)
- Barry Scott (383631)
Publication date
February 2019
DOI Publisher
Public Library of Science (PLoS)Abstract
YTK12 strains transformed with pSUC2T7M13ORI encoding SUC2 transcriptionally fused to gpiB, sspM, sspN or sspO secretion signal nucleotide sequence, as well as the empty vector and YTK12-only controls. Only strains expressing the secretion signal-Suc2 fusion proteins were able to grow on defined medium with raffinose as carbon source (CMDRAA). SS, secretion signal. All strains grew on the control plates containing YPDA media.</p
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