Surface Plasmon Resonance analysis of the interaction between human CD96 and nectin-1 in real time.

Nectin-1(346t) (A) or nectin-1(143t) (B) were immobilized on a CM5 biosensor chip. Various concentrations of hCD96t were flowed over the chip for 2 min (association) and then replaced by buffer for another 2 min (dissociation). Sensorgrams of corrected data are represented after the subtraction of signal from the control flow cell without immobilized nectin-1. Data points were collected at 5 Hz and are represented in color for each of the indicated concentrations of hCD96t. The black lines show the best fit obtained after global fitting with the BIAevaluation 3.0 software using a Langmuir 1:1 model with drifting baseline.