Additional file 2: of SHP1 and SHP2 inhibition enhances the pro-differentiative effect of phorbol esters: an alternative approach against acute myeloid leukemia

Figure S1. Chemical inhibition of SHP1 and SHP2 favors the differentiation of HEL cells. Levels of CD41 and CD61 surface markers in HEL cells 48 h after 4h incubation with the indicated inhibitors of SHP1 and SHP2. Figure S2. Chemical inhibition of SHP1 and SHP2 potentiates morphological changes of PMA in HL-60 cells. Representative pictures of May-Grünwald-Giemsa stained cytospins of HL-60 cells after 48 h treatment with PRS, NSC and their combination. Scale bar: 10 μm. Figure S3. AML cell lines are differentially responsive to PRS and NSC. A) Dose-response curves of HL-60 cells treated with PRS and NSC during 48 h. B) Dose-response curves of AML cell lines different from HL-60 treated with PMA and NSC during 48 h. (DOCX 509 kb