ImageJ intensity plots confirmed cortex-to-membrane positions throughout bleb formation of a putative bleb.

Merges of GFP and RITC time points representative of steps in bleb formation (T0-T3) with their respective intensity plots (dashed and solid lines) from the same cell in Fig 2 confirmed bleb formation quantitatively in real-time along the line drawn (white). Cortex positions (white arrowhead), the actin scar characteristic (black arrowhead), and membrane (black arrow) positions are indicated.