MOESM5 of Updating the modified Thompson test by using whole-body bioluminescence imaging to replace traditional efficacy testing in experimental models of murine malaria

Additional file 5. Limit of detection of the bioluminescence signal in ICR-CD1 study mice infected with luciferase-expressing Plasmodium berghei parasites. Female ICR-CD1 mice infected with 1 × 105 luciferase-expressing P. berghei infected erythrocytes were injected IP with 200 mg/kg luciferin. Whole-body bioluminescence as well as flow cytometry parasitaemia measurements were taken simultaneously. The bioluminescence signal represents the light intensities over the body surface area. Red and bright yellow represent the most intense signal, followed by green and then blue, which represents the weakest signal. The 20 mice showed in this figure had no detectable parasitaemia measured through flow cytometry. Luciferase-expressing P. berghei parasites were visible in the tails of eight ICR-CD1 mice