MOESM4 of Updating the modified Thompson test by using whole-body bioluminescence imaging to replace traditional efficacy testing in experimental models of murine malaria

Additional file 4. Luciferase-expressing Plasmodium berghei infected erythrocytes are present in the blood and brain of female ICR-CD1 mice infected with P. berghei parasites. Female ICR-CD1 mice infected with 1 × 105 luciferase-expressing P. berghei infected erythrocytes were injected IP with 200 mg/kg luciferin 10 min before analysis. Whole-body luminescence measurements were taken 10 min post luciferin injections in 10 ICR-CD1 mice. Mice were euthanized immediately thereafter, brains were harvested, and bioluminescence signal was measured. Additional figure 4A shows the bioluminescence signal in the bodies and brains of 5 mice respectively before and after they were euthanized because of severe malaria. Additional figure 4B shows bioluminescence signal in the bodies and brains of 5 mice that belonged to the positive control (mefloquine) treated group respectively before and after they were euthanized on day 31 post infections which is the last day in the study. The photon intensity numbers in the brighter areas of the image (red or bright yellow) are greater than those in the areas with a dimmer green or blue color, where fewer photons were detected