Rescued DNA replication block and viral yield for ΔB1mutB12 virus in CV1 cells.

(A) CV1 cells infected with WT (black), ΔB1 (red), ΔB1mutB12-A1 (light green), ΔB1mutB12-A3 (dark green) at a MOI of 3 were harvested 24h post infection for qPCR of relative DNA accumulation or (B) for titration on CV1-B1myc cells for viral yield. (C) WT (black), ΔB1 (red), or ΔB1mutB12-A3 (green) infections of CV1 cells were performed at a MOI of 3 and harvested at 3, 7, 16, or 24h post infection for relative DNA accumulation or (D) viral yield quantification on CV1-B1myc cells. (E) A multi-step viral yield assay was completed by infecting CV1 cells at a MOI of 0.01 with WT (black), ΔB1 (red) or ΔB1mutB12-A3 (green) and harvested at 48h post infection for titration on CV1-B1myc cells.