Dynein is required for injury-induced Schwann cell morphology changes.

(A-F) Schwann cells in 5 dpf larvae labeled by Tg(sox10:mRFP). (A) Prior to injury, wild type Schwann cells have smooth, straight membranes that are tightly associated with the axonal track (scale bar = 5 μm). (B) After axonal fragmentation, Schwann cell membranes change morphology and widen to accommodate axonal debris. (C) Prior to injury, gpr126-/- Schwann cells are loosely associated with axons as they do not myelinate. (D) After axonal fragmentation, gpr126-/- Schwann cells are able to change morphology and widen. (E) Prior to injury, dync1h1-/- Schwann cells are loosely associated with axons as they also do not myelinate. (F) After axonal fragmentation, dync1h1-/- Schwann cell membranes maintain an elongated conformation and do not dramatically change morphology, indicating a disrupted injury response. (G) Quantification of Schwann cell width pre- and post-fragmentation in gpr126 and dync1h1 mutants.