Dynein stabilizes axonal extensions during regeneration.

(A-B) In wild type animals, regenerating axons begin probing the environment by extending and retracting (green and red arrowheads, respectively; scale bar = 5 μm). (C-D) dync1h1-/- axons also extend and retract after injury. (E) Quantification of extension and retraction events in wild type siblings (n = 13 axons) and dync1h1-/- axons (n = 13 axons). (F-G) Measurement of overall growth cone displacement from transection site ~16 hpt in wild type siblings (F; blue arrowheads, growth cones; scale bar = 10 μm) and dync1h1-/- (G; red arrowheads, growth cones). (H) Quantification of growth cone displacement ~16 hpt (wild type siblings, n = 15; dync1h1-/-, n = 10; p = 0.0005, unpaired t-test).