Additional file 3: of Regulation of cyclin T1 during HIV replication and latency establishment in human memory CD4 T cells

Generation of p24/CycT1 cells during HIV replication in modestly stimulated memory CD4 T cells. Memory CD4 T cells were purified from peripheral blood and uninfected or infected with HIV (R5 strain SF162) in IL2 medium for 2 days. Cells were washed and cultured with either 0.5 μg/ml CD3 mabs alone or 0.1 μg/ml IL2 alone for 6 days. Cells were then stained for p24, CycT1, CD69, and CD25. (A-B) Shown are sample dotplots and mean ± sem p24+CycT1- and p24+CycT1+ cells gated on CD69/CD25 populations (*p < 0.05, N = 3). (PPTX 443 kb