Additional file 2: of Regulation of cyclin T1 during HIV replication and latency establishment in human memory CD4 T cells

Cyclin T1 expression in small and large memory CD4 T cells during T cell activation. Human CD4+CD45RO+ memory T cells were purified from peripheral blood and cultured without (No Costimulation) or with CD3 + CD28 mabs and IL2 (Costimulation) for 5 days, then stained for CycT1, CD69, CD25, HLA.DR, and CD38. (A) Shown are sample Isotype-FITC or CycT1-FITC dotplots gated on overall, small, or large cells, and (B) mean ± sem CycT1, CD69+CD25+, or HLA.DR+CD38+ expression (N = 5). (PPTX 595 kb