Steady-state abundance of maternal miRNAs predicted to target specific stress transcripts in eggs derived from 5 and 9 months old zebrafish.

An n = 8 samples of pooled eggs extracted from a single female fish (CTL and FLX groups) were used to quantify miRNA-740 (A), miR-25 (B), miR-26a (C), miR-30d (D), miRNA-92a (E) and miRNA-103 (F). Data are normalized to respective control groups at 5 and 9 months, and expressed as fold-change. Data were analyzed using t-test or Mann-Whitney U test, and significant differences are indicated by different letters within each sampling timepoint.