ICC results of Elk1 and Elk1-3R effects on endogenous katanin-p60 level.

Asterisk symbol represents untransfected cells, whereas the cells indicated with arrows are either Elk1 or Elk1-3R overexpressing cells. The images were taken using 63X objective at zoom 2.6. (A) The level of endogenous katanin-p60 protein is reduced in Elk1 overexpressed SH-SY5Y cells compared to untransfected cells. (B) Relative fluorescence density graph shows that the expression of katanin-p60 was 5.5 fold decreased in Elk1 overexpressed cells compared to control cells. SEM values are 0 and 0.02, respectively. (C) The decrease in endogenous katanin-p60 level is observed in Elk1-3R overexpressed SH-SY5Y cells compared to untransfected cells. (D) Relative fluorescence density graph shows that the expression of katanin-p60 was 4.76 fold decreased in Elk1-3R overexpressed cells compared to control cells. SEM values are 0 and 0.03, respectively.