Trpm4 ion channels in preBötC neurons.

(A) Transverse section from an adult Dbx1;Ai9 mouse showing tdTomato (magenta) expression in Dbx1-derived cells. (B) Selection box from A encompasses the preBötC and shows Trpm4 expression (cyan). (C) Selection box from the preBötC in B showing tdTomato (Dbx1) and Trpm4 expression in preBötC neurons. Trpm4 is expressed in both Dbx1 neurons (arrowheads) and non-Dbx1 neurons (arrow). (D) GFAP expression (yellow) identifies Dbx1-derived astrocytes of the preBötC (yellow and magenta, marked with an asterisk), which lack Trpm4 expression. Arrowhead identifies a Dbx1 neuron with Trpm4 expression. Separate scale bars are shown for A, B, C, and D. Dbx1, developing brain homeobox 1; GFAP, glial fibrillary acidic protein; preBötC, pre-Bötzinger complex; Trp, transient receptor potential.