Trpm4 but not Trpc3 antagonists attenuate preBötC rhythms in vitro.

(A) preBötC field potentials (black) and XII output (gray) in control and at steady state following approximately 20 min application of 100 μM 9-phenanthrol in neonatal CD-1 mice. A typical eupnea-related inspiratory burst is marked with ε; a typical sigh burst is marked with σ. (B–E) Area of inspiratory (B) and sigh (C) bursts as well as inspiratory (D) and sigh (E) frequency in control and 9-phenanthrol. (F) preBötC field potentials (black) and XII output (gray) in control and at steady state following approximately 20 min application of 10 μM pyr-3. Again, ε marks a eupnea-related inspiratory burst; σ marks a sigh burst. (G–J) Area of inspiratory (G) and sigh (H) bursts as well as inspiratory (I) and sigh (J) frequency in control and pyr-3. Mean ± SD shown (magenta) for all graphs in B–D and G–J. Primary data can be found in the Supporting information (S1 Data). preBötC, pre-Bötzinger complex; pyr-3, pyrazole-3; XII, hypoglossal nerve root.