β-Catenin and TCF7L2 bind the rs6651252 locus in colonic epithelial cells.

(A). Diagram of the chromosomal MYC locus. Shown is the position of rs6651252 relative to MYC and PVT1 genes on chromosome 8. Blue rectangles above depict positions of ChIP-Seq peak regions for β-catenin in HCT116 cells. Clustered vertical lines at bottom indicate H3K27 acetylated regions of chromatin that were downloaded from the UCSC Genome Browser, build hg18 (http://genome.ucsc.edu/). (B). qPCR analysis of DNA fragments precipitated with α-β-catenin antibodies in ChIP assays conducted in HCT116 and DLD-1 colonic epithelial cell lines, and in HEK293T cells. Oligonucleotides used in the PCR reactions flanked rs6651252 or a region in TUBULIN as a negative control. (C) and (D)., as in (B) except α-TCF7L2 or α-H3K27Ac antibodies were used in the ChIP assays, respectively. The data are presented as percent of input with error bars representing SEM (**P P <0.001).