A specific commercially available ELISA procedure was used to determine IL10 concentration (pg/mL) in LTT supernatants, which had been immediately stored at -20°C after separation from cells. The IL10 peak is observed earlier in GI (pPAL-LACK/pPAL-canIL12-p35/pPAL-canIL12-p40) than in GII (infection control group). *Several dogs had to be euthanized before the end of the experiment (after the 240dpi time point) for ethical reasons.</p
<p>Box and whisker plot with outliers showing interquartile range (median bar in intersect between t...
<p>The median values of IL-10 production at different time points are tabulated. Freshly isolated PB...
<p>T cell enriched PBMCs were treated with 10, 25, or 100 µg/ml LA for 5 minutes prior to treatment ...
A specific commercially available ELISA procedure was used to determine IFN-γ concentration (pg/mL) ...
<p>Each line represents a different dog, with individual colors representing different treatment gro...
Parasite burden is similar in GI (pPAL-LACK/pPAL-canIL12-p35/pPAL-canIL12-p40 group) and GII (infect...
CD4+-proliferation against the LACK protein is observed only in group GI (pPAL-LACK/pPAL-canIL12-p35...
<p>(A) IFN-γ was quantified at time zero and one month after each dose of rLdccys1. (B) IL-10 was qu...
<p>Spleen was examined for B220, CD4, CD8, and IL-10. Y-axis indicates percentage of B220+, CD4+, ...
Clinical signs were evaluated 150, 180, 240, and 300 dpi. GI: dogs which received two doses of 200μg...
Copyright © 2013 Nathalee Prakash et al. This is an open access article distributed under the Creati...
<p>Dogs were infected with <i>Leishmania infantum-chagasi</i> plus five pairs of <i>Lutzomyia longip...
<p>IHC images of IL-10 in healthy (score≤1.5) ileal sections from DF, Control, and BCFA pups. IL-10 ...
<p>Measurement of cytokines in peripheral blood indicated greater concentrations of pro-inflammatory...
<p><b>A</b>: Control animals (n = 7) received normal sterile saline (i.p.; 0.5 ml) after collection ...
<p>Box and whisker plot with outliers showing interquartile range (median bar in intersect between t...
<p>The median values of IL-10 production at different time points are tabulated. Freshly isolated PB...
<p>T cell enriched PBMCs were treated with 10, 25, or 100 µg/ml LA for 5 minutes prior to treatment ...
A specific commercially available ELISA procedure was used to determine IFN-γ concentration (pg/mL) ...
<p>Each line represents a different dog, with individual colors representing different treatment gro...
Parasite burden is similar in GI (pPAL-LACK/pPAL-canIL12-p35/pPAL-canIL12-p40 group) and GII (infect...
CD4+-proliferation against the LACK protein is observed only in group GI (pPAL-LACK/pPAL-canIL12-p35...
<p>(A) IFN-γ was quantified at time zero and one month after each dose of rLdccys1. (B) IL-10 was qu...
<p>Spleen was examined for B220, CD4, CD8, and IL-10. Y-axis indicates percentage of B220+, CD4+, ...
Clinical signs were evaluated 150, 180, 240, and 300 dpi. GI: dogs which received two doses of 200μg...
Copyright © 2013 Nathalee Prakash et al. This is an open access article distributed under the Creati...
<p>Dogs were infected with <i>Leishmania infantum-chagasi</i> plus five pairs of <i>Lutzomyia longip...
<p>IHC images of IL-10 in healthy (score≤1.5) ileal sections from DF, Control, and BCFA pups. IL-10 ...
<p>Measurement of cytokines in peripheral blood indicated greater concentrations of pro-inflammatory...
<p><b>A</b>: Control animals (n = 7) received normal sterile saline (i.p.; 0.5 ml) after collection ...
<p>Box and whisker plot with outliers showing interquartile range (median bar in intersect between t...
<p>The median values of IL-10 production at different time points are tabulated. Freshly isolated PB...
<p>T cell enriched PBMCs were treated with 10, 25, or 100 µg/ml LA for 5 minutes prior to treatment ...