FH535 regulates autophagic activity in HCC cells.

Western blot analysis of Huh7 cell after 40 h treatment with FH535 at indicated concentrations in absence (-CQ) or presence (+CQ) of 50 μM chloroquine for 8h. LC3B (A) and p62 (B) were used as autophagy markers for western blot analysis. Band intensity were estimated using ImageJ software. Autophagic flux was determined by subtracting the band intensity of LC3B II western blot in presence of CQ and the corresponding treatment in absence of CQ which is referred as ΔLC3II (LC3II (+CQ)—LC3II (-CQ)) (A, right panel). mRNA p62 expression levels were assessed by RT-qPCR in absence of CQ (C).