FH535-N regulates autophagic activity in HCC cells.

Western blot analysis of Huh7 cell after 40 h treatment with FH535-N at indicated concentrations in absence (-CQ) or presence (+CQ) of 50 μM chloroquine for 8h. (A) Protein expression levels of LC3BII. Autophagic flux was determined by subtracting the band intensity of LC3B II western blot in presence of CQ and the corresponding treatment in absence of CQ which is referred as ΔLC3II (LC3II (+CQ)—LC3II (-CQ)). (B) Protein expression levels of p62 in absence of CQ. Band intensity from Western blots were estimated using ImageJ software.