Oligomerization of hTRPA1 coiled-coil.

(A) Cartoon depicting the MBPCC1 fusion protein along with Coomassie-stained gels of purified MBPCC1, MBPCC2, and isolated CC1 protein. (B) SEC-MALS showing high concentration of MBPCC1 forming a tetramer (Mw calculated to be 203 kDa +/- 1.5%) in the absence of IP6. (C) SEC-MALS showing MBPCC1 at lower concentration is no longer tetrameric but containing a mix of trimers (Mw calculated to be 136 kDa) and monomers (Mw calculated to be 53 kDa) in the absence of IP6. For panels in (B) and (C), blue dots indicate absorbance at A280 and black or red dots indicate regions and values where Mw was calculated. (D), (E). SEC chromatograms of MBPCC1, MBPCC2, and a mixture of MBPCC1+MBPCC2 in the absence (D) or presence (E) of IP6 showing no clear signs of interaction between CC1 and CC2.