Additional file 1: of Induction and decay of functional complement-fixing antibodies by the RTS,S malaria vaccine in children, and a negative impact of malaria exposure

Induction and decay of functional complement-fixing antibodies by the RTS,S vaccine in children and a negative impact of malaria exposure supplementary material. Supplementary equation 1. Supplementary methods for recombinant C-terminal region of CSP (Figure S1.). Supplementary methods for C1q-fixation assay (Figure S2.). Figure S3. RTS,S predominately induces anti-CSP IgG1 and some IgG3, IgG2, and IgM antibodies. Figure S4. Epitope specificity of children with high or low C1q-fixing antibodies. Figure S5. Younger and older children in Manhiça and Ilha Josina cohorts. Figure S6. Functional complement-fixing antibodies decline over time. Figure S7. Antibody concentration and functional C1q-fixation responses. Table S1. Linear regression between epitope-specific IgG and C1q-fixation to CSP, induced by vaccination with RTS,S (N = 99). (DOCX 2573 kb