The C-terminal region of Net1 is an activator of RNA polymerase I transcription with conserved features from yeast to human - Fig 6

The CTR of Net1 interacts with many chromosomal loci and rescues slow growth in net1Δctr strains when fused to Rpa190. (A, B) Haploid yeast strains (y3087; y3157; y3160) carrying a NET1 or a net1Δctr allele and expressing CTR-MNHA or Net1-MNHA were subjected to ChEC analyses, as described in the legend to Fig 4. B) Agarose gels were stained with SYBR safe before transfer to the blotting membrane (upper panels), sizes of selected DNA fragments in the DNA marker (M) are given on the left. Lower panels, the membrane was hybridized with a radioactively labeled probe hybridizing with a XcmI fragment containing the NUP57/RPS23A loci as indicated in the cartoon on the left. C, D) Haploid yeast strains (y3673; y3674; y3746; y3747; y3748; y3749; y3756; y3757; y3758; y3759, y3290, y3298) carrying a NET1 or a net1Δctr allele and expressing CTR fusion proteins of Rpa190, or Rrn7 or the wild-type proteins, were subjected to growth analyses in liquid cultures (C), and on solid medium (D) as described in the legend to Fig 2A and 2B.