Figure S2. CXCL12-CXCR4 chemotaxis mediates mast cell migration and accumulation in GC tumors. (a) Expression of Ki-67 in tumor-infiltrating mast cells by gating on CD45+CD117+FcεRI+ cells. Color histograms represent staining of Ki-67; black, isotype control. (b) Tumor-infiltrating tryptase+ mast cells and Ki-67+ cells were defined by immunofluorescence staining. Green, Tryptase; red, Ki-67; and blue, DAPI-stained nuclei. Scale bars: 50 μm. (c) Expression of CCR2, CCR4, CCR5, CCR7, CXCR1, CXCR2 and CXCR7 on tumor-infiltrating mast cells by gating on CD45+CD117+FcεRI+ cells. Color histograms represent staining of chemokine receptors; black, isotype control. (d) Representative analysis of CXCL12-expressing (red) EpCam+ tumor cells (green) in ...
Figure S1. Tregs infiltration in HT29 spheroids. Percentages of Foxp3+CD25+ Tregs among CD4+ T cells...
Additional file 2: Figure S2. Expression of inhibitory molecules Tim-3+ and PD-1+ on CD8+. (A) Flow ...
Abstract Background Mast cells are prominent components of solid tumors and exhibit distinct phenoty...
Figure S3. Tumor-derived factor TNF-α induces mast cells to express PD-L1. (a) Expression of 2B4, gl...
Figure S5. Tumor-infiltrating and tumor-conditioned mast cells suppress T cell immunity through PD-L...
Figure S4. Tumor-derived TNF-α activates NF-κB pathway to induce PD-L1 expression on mast cells. (a)...
Figure S7. Kaplan-Meier plots for disease-free survival by median mast cell percentage (9.315%) or m...
Table S2. Clinical characteristics of 114 patients with gastric cancer. (DOCX 19 kb
Figure S1. Mast cell percentage (a) or mast cell number (b) and its potential correlations with clin...
Table S5. Correlations between mast cell number and clinic pathological features of patients with ga...
Table S4. Correlations between mast cell percentage and clinic pathological features of patients wit...
Figure S6. Blockade of mast cell-associated PD-L1 on T cell immunity inhibits tumor growth and GC pr...
Figure S1. Gastric cancer cell-derived conditioned medium induced pro-inflammatory factor gene expre...
Figure S1. Representative staining of ILC populations. ILC were gated on lineage negative, CD45+, CD...
Figure S1. Representative photos of TG2-overexpressing MCF7 cells (MCF7_TG2) and control vector-tran...
Figure S1. Tregs infiltration in HT29 spheroids. Percentages of Foxp3+CD25+ Tregs among CD4+ T cells...
Additional file 2: Figure S2. Expression of inhibitory molecules Tim-3+ and PD-1+ on CD8+. (A) Flow ...
Abstract Background Mast cells are prominent components of solid tumors and exhibit distinct phenoty...
Figure S3. Tumor-derived factor TNF-α induces mast cells to express PD-L1. (a) Expression of 2B4, gl...
Figure S5. Tumor-infiltrating and tumor-conditioned mast cells suppress T cell immunity through PD-L...
Figure S4. Tumor-derived TNF-α activates NF-κB pathway to induce PD-L1 expression on mast cells. (a)...
Figure S7. Kaplan-Meier plots for disease-free survival by median mast cell percentage (9.315%) or m...
Table S2. Clinical characteristics of 114 patients with gastric cancer. (DOCX 19 kb
Figure S1. Mast cell percentage (a) or mast cell number (b) and its potential correlations with clin...
Table S5. Correlations between mast cell number and clinic pathological features of patients with ga...
Table S4. Correlations between mast cell percentage and clinic pathological features of patients wit...
Figure S6. Blockade of mast cell-associated PD-L1 on T cell immunity inhibits tumor growth and GC pr...
Figure S1. Gastric cancer cell-derived conditioned medium induced pro-inflammatory factor gene expre...
Figure S1. Representative staining of ILC populations. ILC were gated on lineage negative, CD45+, CD...
Figure S1. Representative photos of TG2-overexpressing MCF7 cells (MCF7_TG2) and control vector-tran...
Figure S1. Tregs infiltration in HT29 spheroids. Percentages of Foxp3+CD25+ Tregs among CD4+ T cells...
Additional file 2: Figure S2. Expression of inhibitory molecules Tim-3+ and PD-1+ on CD8+. (A) Flow ...
Abstract Background Mast cells are prominent components of solid tumors and exhibit distinct phenoty...