RNAi analysis of the <i>C</i>. <i>elegans</i> Integrator complex.

(a) RNA deep sequencing reads aligned to the C. elegans genome in the region of the U1 snRNA: H27M09.8 and the downstream in sense gene H27M09.5 visualized using IGV software for each RNAi Integrator member knockdown (logarithmic scale). L4440 control reads are shown in gray whereas Integrator complex RNAi reads are in black. Underneath each graph, the C. elegans genome is represented in blue. The exons are shown as blue boxes and the introns as lines. (b) Phenotypes of C. elegans Integrator complex predicted subunits after RNAi knockdown. L1 stage N2 worms were fed bacterial RNAi clones of each predicted member of the Integrator complex. Worms were grown at 15°C. Images were taken on the sixth day (adult stage). ints-2, -4, -5, -9, -11 show a larval arrest phenotype (lva), ints-1, -6, -7, -8 reached adulthood but had reduced offspring that died embryonically (emb) whereas ints-3, -10, -12, -13 showed non-obvious phenotypes.