<i>C</i>. <i>elegans</i> Integrator complex identification.

(a) Predicted C. elegans Integrator complex orthologs versus H. sapiens Integrator complex subunits. Protein domains were searched using the Pfam database. The length of each subunit is indicated in amino acids (aa). Abbreviations: DUF, domain of unknown function; HEAT, Huntingtin, Elongation factor 3, protein phosphatase 2A, and the yeast kinase TOR1; ARM, armadillo-like repeats; COIL, coiled coil domain; VWA, von Willebrand type A domain. β-lactamase/β-CASP (*indicates the presence of an inactive β- lactamase/β-CASP domain); RMMBL, Zn-dependent metallo-hydrolase, RNA specificity domain; 7TM GPCR srh: seven-transmembrane G-protein-coupled receptor, serpentine receptor class h; PHD, plant homeodomain finger; ISDCC, INTS6/SAGE1/DDX26B/CT45 C- terminus; TPR, tetratricopeptide repeats. (b) INTS-6 Immunoprecipitation scheme. Protein extracts were obtained from the JCP378 strain. The majority of the immunoprecipitate was analysed by LC-MS/MS. In addition, a small amount of the immunoprecipitate was run on SDS-PAGE and silver stained. The Integrator complex members detected by mass spectrometry are indicated on the silver stained gel according to their expected molecular weight. (c) Polypeptides detected from the Integrator complex predicted the orthologs in the four independent INTS-6 co-immunoprecipitation analyses.