<i>pde</i> mutants display ectopic melanocytes and iridophores, but not xanthophores, in the ventral medial pathway.

(A,B) Overview of early larval WT (A) and pde (B) pigment phenotype at 5 dpf. (C-F) Anatomical location of ectopic pigment cells in pde. Magnification of lateral views (white boxes in A and B) and cross sections of posterior trunks show no pigment cells on the medial migration pathway in the ventral trunk of WT larvae (C and D). Ectopic pigment cells are located in the ventral trunk of pde mutants (E; red arrowhead), under the dorsal aorta (DA) and above the posterior cardinal vein (PCV) as shown by cross sections (F, white arrowhead). (G-L) Whole mount in situ hybridization of 3 dpf WT (G-H) and pde mutants (J-L) embryos for dct (G and J), ednrba (H and K), and gch (I and L). Ectopic dct (J; white arrowhead) and ednrba (K; white arrowhead) expression is seen in the ventral trunk of pde mutant larvae. Neural tube (NT), notochord (NC) and ventral stripe (VS). Scale bar = 500 μm (A and B) and 100 μm (C and F) and 50 μm (G-L).