Collagen 1 and 3 mRNA expression levels determined by RT-PCR in non-injured omentum.
- Verónica Gómez-Gil (5925128)
- Marta Rodríguez (292472)
- Francisca García-Moreno Nisa (6407627)
- Bárbara Pérez-Köhler (292473)
- Gemma Pascual (292470)
Publication date
February 2019
DOI Publisher
Public Library of Science (PLoS)Abstract
Relative mRNA levels of collagen 1, collagen 3 and Col1/Col 3 ratios in samples of omentum (non-injured and not involved in adhesions) from animals implanted with different meshes. Gene expression was normalized to the expression recorded for the reference gene GAPDH. Agarose gel electrophoresis of RT-PCR products is shown: P, Preclude; S, Surgipro; D, DynaMesh; T, TiMESH. Mw: Molecular weight markers. N: Negative. All reticular meshes showed statistically significant higher collagen 1 mRNA expression levels compared to the laminar Preclude (**, p<0.01 vs. Surgipro and *, p<0.05 vs. DynaMesh, TiMESH). Col 1/ Col 3 ratios were greatest in TiMESH vs. Preclude or Surgipro (*, p<0.05).</p
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