Peptide stimulation of splenocytes from apoE-/- mice subjected to MI.

Splenocytes from male apoE-/- mice 6 weeks after MI were stimulated with individual peptides for 24 hours. Cells from mice not subjected to surgery (Cont) or subjected to sham surgery were compared to mice subjected to MI. Cells were harvested and stained for flow cytometry for CD8+ (A and B) or CD4+ (C and D) EM and CM T cells. Gating strategy is as indicated in Fig 3. Bar over control and peptide treated column indicates statistical significance within respective group (Control P<0.01; Sham P<0.05; MI P<0.01). Spleens from 2–3 mice were pooled per group, N = 3 each. No Tx = no peptide treatment; Ker II = Keratin, type II; BH = Bleomycin Hydrolase.