Pollen aperture phenotypes and INP1-YFP localization in the <i>mcr</i> and <i>dnt</i> mutants.

(A-A’) mcr pollen has a single ring-like aperture (arrowheads) that runs around the pollen circumference (front and back views of the same pollen grain are shown). See also S2 Video. (B-B’) dnt pollen usually has two round apertures (arrowheads) located at diametrically opposite positions (front and back views of the same pollen grain are shown). See also S3 Video. Scale bars = 10 μm. (C) A 3D reconstruction of a tetrad of mcr microspores with early exine (blue) and single ring-like apertures (arrowhead) visible. Each aperture runs through the poles of microspores. (D-F) INP1-YFP localization changes in the mcr and dnt mutants. (D) INP1-YFP forms a single punctate line in mcr microspores, passing through the microspore poles and underlying the position of the future single ring-like aperture. See also S4 Video. (E) INP1-YFP localization to two sites close to each microspore equator indicates that the round apertures in mcr start their development as two apertures that eventually fuse at the poles. See also S5 Video. (F) In the dnt mutant tetrads, INP1-YFP forms punctate circles at the proximal and distal poles of each microspore. See also S6 Video.