Comparison of codons in transcripts versus tRNA count in genomic assembly.

The relative synonymous codon usage (RSCU) for 28 codons in 1,466 transcripts are shown as a bar graph in the bottom portion, while the top portion shows number of complementary tRNA found in the genome survey. The black bars are used for codons ending in G or C, while the grey bars are for codons ending in A or U. The codons were sorted based on third positions ending in C or U (left portion of figure) when compared with those ending in G or A (right portion). Due to redundancy in the genetic code some amino acids have between one and six possible codons. The 28 codons shown include the 16 third position informative codons ending in C or T and A or G, or amino acids with just two codons. For comparison an additional 12 codons for amino acids with three to six codons are shown and indicated with an asterisk. The RSCU values for likely glutamine codons (UAG, UAA and CAU, CAG) sum to four while phenylalanine with two codons would sum to two and serine to six. All of the codons that were synonymous at third positions and AUG, AUA, UGG and UGA were excluded.