Np9 is essential for cell migration and wound closure.

(A) NCCIT scramble control and NCCIT Np9 KD clones 8 and 9 were used for the migration assay. A total of 200,000 cells for each cell type were transferred into a 12-well plate. The following day, a scratch was created down the middle, and pictures were taken at time 0 and at 24 hours. The images are representative of 3 independent experiments done in duplicate. Scales bars, 100 μm. (B) The wound was measured at time 0 and at 24 hours. The percent of migration was plotted over time 0. The measurements are representative of 3 independent experiments done in duplicate. The width of the wounds was measured in each image and totaled for scramble CT (n = 26), KD #8 (n = 27), and KD #9 (n = 28). The reduction in the expression of Np9 in NCCIT cells resulted in reduced cell migration post-wound as compared to scramble control cells. T bars denote the standard deviations of the means; P values were determined using student t-test analysis. ***P<0.0001.