Additional file 5: of Oxygen induces the expression of invasion and stress response genes in the anaerobic salmon parasite Spironucleus salmonicida

Figure S3. Phylogenetic analysis suggests proteins related to oxygen stress response were acquired by lateral gene transfer. Phylogenetic trees were generated for (A) NADH oxidase, (B) Flavodiiron protein, (C) Superoxide reductase and (D) Rubrerythrin. Maximum likelihood (ML) phylogenies were generated using IQTREE under the indicated model of evolution. For visualization purposes, distantly related clades of prokaryotes were collapsed. Complete phylogenies can be found in Additional file 4 and [130]. Bipartition values from 1000 ultrafast bootstrap replicates were mapped onto the best scoring ML tree and labeled with a solid or open circle to represent bipartition values greater than 95 or 80 respectively. Bipartitions supporting diplomonad monophyly or sister relationship to prokaryotes are shown in grey circles and squares respectively. Genes that were significantly up or down regulated are indicated for OXY (left) and NAO (right) cells with up or down arrow respectively. Organisms are colored based on their taxonomic classification, eukaryotes (green), metamonads (light purple), fornicates (dark purple), archaea (orange) and bacteria (black). (TIF 4932 kb