Impact of 8-MOP concentration (0 ng/ml, 200 ng/ml, or 300 ng/ml) on cellular response after a 2 J/cm² UVA dose.

(A) Percentage of Annexin-V+ cells measured daily up to 2 days after irradiation. (B) Δapoptosis determined on days 1 and 2 post-irradiation. (C) Fold increase on day 3 post-irradiation relative to day 0. (D) Proliferation inhibition percentage, calculated as the percentage decrease in proliferation in irradiated versus control samples. (E) Residual 8-MOP remaining in the irradiation container after treatment, expressed as a percentage of the pre-irradiation 8-MOP concentration. For clarity, only statistically significant results are shown.*p<0.05; **p<0.01; ***p<0.001. Significant differences with respect to the control (UVA -) are indicated with an asterisk above the relevant bar, while comparisons between irradiated samples are indicated with an asterisk over a line linking the relevant bars. For Annexin-V+ cells (A) and Δapoptosis (B), comparisons performed on days 1 and 2 post-irradiation are indicated in black and gray, respectively.