Rad51-GFP is not dominant negative.

A) Schematic of repair by gene conversion in YJK17. An HO-induced DSB made on chromosome III is repaired by the MATa-inc donor sequence on chromosome V, which contains a mutation (-inc) that prevents further cutting. B) Percent viable cells following HO induction and repair through ectopic gene conversion in the indicated derivative of YJK17. Error bars represent the SD of three biological replicates. C) Percentage of cells displaying a focus in the indicated derivative of YJK17 at the indicated time. Error bars represent the SD of three biological replicates of >150 cells per experiment. D) qPCR analysis of the timing of DSB repair by gene conversion in the indicated derivatives of YJK17. E) Rad51 protein abundance ascertained by chromatin immunoprecipitation 250 bp distal to the HO cleavage site at MAT. Error bars represent SEM of three individual experiments performed in triplicate F) Fluorescence nuclear intensity of cells measured in (C) G) Measured intensity of the Rad51-GFP focus 3 h after HO induction in the indicated strain. Box and whisker plots prepared as in Fig 1D. H) Spot dilution assay without and with 0.002% MMS.