Mice are motivated but not water restricted.

(a) Number of trials performed normalised to mean of trials performed across animals at 100% reward vs. the amount of reward delivered on each correct trial (n = 4 mice, 100% reward = 12μl). Mice perform fewer trials with larger reward volumes (roughly according to the red line of constant daily water intake (red: trials x reward = const line)). (b) Number of trials per vs. recorded day’s weight in a separate cohort (n = 20). There is a strong positive correlation between weight and number of trials performed, suggesting that animals are capable of regulating their own metabolic demands within AutonoMouse.