Overexpression of PEDV N enhances viral sgmRNA transcription.

Vero E6 or Vero E6-N cells were infected with PEDV-AVCT12-mCherry (MOI = 0.0001) and analyzed at indicated time post-infection. For RNA analysis, total RNAs were extracted and treated with DNaseI prior to RT-qPCR experiments with primer pairs specific to ORF1 for viral RNA genome quantification (A) or to sgmRNA of S for transcription (B). Values are averages±SEM of three independent experiments. * p p < 0.01. For protein analysis, lysates were analyzed with western blotting with anti-S1 antibody (C).