Kinetics of viral protein accumulation in turnip leaves.

Total extracts of samples taken from the same systemically infected leaf at the time points indicated were analyzed by Western blotting with antisera against P2, P3, P4 or P6 (indicated by red arrows). The first panel (RuBisCO) shows a loading control (Ponceau Red staining of the large chain RuBisCO subunit). It should be noted that under the electrophoresis conditions used, much of the capsid protein P4 did not enter the gel properly and was retained in the upper part of the gels (blue arrow). The three red arrows in the anti-P4 blot point to the various mature P4 forms detected in infected plants [25]. Shown are the representative Western blots from one experiment out of seven performed.